Optimum Culture Conditions for Production of Exfoliative Toxin byStaphylococcus hyicus
نویسندگان
چکیده
منابع مشابه
Chromosomal and extrachromosomal synthesis of exfoliative toxin from Staphylococcus hyicus.
Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless strain P-1 (SHETA) and from plasmid-carrying strains P-10 and P-23 (SHETB) we...
متن کاملProduction of exfoliative toxin by isolates of Staphylococcus hyicus from different countries.
A total of 218 isolates of Staphylococcus hyicus from pigs in eight countries (Belgium, Croatia, Germany, Japan, Korea, Slovenia, the uk and the usa) and 44 isolates from other animals in Belgium, India, Japan and the usa were examined for the genes encoding the exfoliative toxins ExhA, ExhB, ExhC and ExhD by multiplex pcr. The expression of the toxins was confirmed by immunoblot analysis, usin...
متن کاملNew exfoliative toxin produced by a plasmid-carrying strain of Staphylococcus hyicus.
A new serotype of Staphylococcus hyicus exfoliative toxin (SHET), serotype B, was isolated from the culture filtrate of a plasmid-carrying strain of S. hyicus. The new SHET was purified by precipitation with 70% saturated ammonium sulfate, gel filtration on a Sephadex G-75 column, column chromatography on DEAE-Cellulofine A-500, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS...
متن کاملDevelopment and evaluation of an indirect ELISA for detection of exfoliative toxin ExhA, ExhB or ExhC produced by Staphylococcus hyicus.
Immunoblot analysis and enzyme-linked immunosorbent assay (ELISA) confirmed previous reports that the Staphylococcus hyicus exfoliative toxins ExhA and ExhB are metalloproteins, and further indicated that ExhC is also a metalloprotein. An indirect ELISA was developed for the detection of toxigenic strains as an alternative method to the use of phage typing for selection of S. hyicus isolates to...
متن کاملStaphylococcus hyicus exfoliative toxins selectively digest porcine desmoglein 1.
Virulent strains of Staphylococcus hyicus can cause exudative epidermitis in pigs. The major symptom of this disease is exfoliation of the skin in the upper stratum spinosum. Exfoliation of the skin is strongly associated with exfoliative toxin including ExhA, ExhB, ExhC, ExhD, SHETA, and SHETB. Recently, genes for ExhA, ExhB, ExhC and ExhD were cloned. Exfoliative toxins produced by S. aureus ...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1995
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1995.tb02215.x